All of our DNA template controls are adjusted to a final concentration of 200 ug/ml in 1/10 TE (1mM Tris-HCl (pH8.0), 0.1mM EDTA). This diluent provides sufficient buffering capacity and EDTA to protect the DNA controls without interfering with the Mg+2 concentrations required for robust PCR amplification.

Locus/Target
These DNA template controls are characterized to have the listed gene rearrangement, gene translocation, or gene mutation.
IGH Gene Rearrangement Controls
IGK Gene Rearrangement Controls
IGL Gene Rearrangement Controls
TCRB Gene Rearrangement Controls
TCRD Gene Rearrangement Controls
TCRG Gene Rearrangement Controls
BCL1/JH t(11;14) Translocation Controls
BCL2/JH t(14;18) Translocation Controls
MLL/AF4 t(4;11) Translocation Controls
FLT3 Gene Mutation Controls

Polyclonal DNA
Human Polyclonal Genomic DNA from Tonsil

All DNA Controls
IVS-0000 - From Human Tissue
IVS-0001 to IVS-0009 - From Cell Lines
IVS-0010 to IVS-0019 - From Cell Lines
IVS-0020 to IVS-0029 - From Cell Lines
IVS-0030 to IVS-0039 - From Cell Lines
IVS-P001 to IVS-P003 - From Plasmids

Plasmid DNA controls & calibrators
IVS-P001 Clonal Control DNA
IVS-P002 Clonal Control DNA

All of our RNA template controls are adjusted to a final concentration of 400 ug/ml in RNase-free, glass-distilled water. The pH of distilled water is slightly acidic; this protects the RNA from hydrolysis.

Locus/Target
These RNA template controls are characterized to have the listed gene rearrangement, gene translocation, or gene mutation.

BCR/ABL t(9;22) Translocation Controls
PML/RARa t(15;17) Translocation Controls
CBFB/MYH11 inv(16) Control
E2A/PBX1 t(1;19) Translocation Controls
AML1/ETO t(8;21) Translocation Control

All RNA Controls
IVS-0001 to IVS-0009 - From Cell Lines      
IVS-0010 to IVS-0019 - From Cell Lines      
IVS-0020 to IVS-0029 - From Cell Lines      
IVS-0030 to IVS-0039 - From Cell Lines          

The BCR/ABL Lyophilized Cell Controls are manufactured from BCR/ABL expressing cells that have been diluted 1:10 (10^-1) and 1:1000 (10^-3) in a background of cells that do not express the BCR/ABL transcript.  These cell mixtures, as well as a negative control, are suspended in 2X PBS prior to lyophilization.  Each panel member contains 1.0×10⁶ cells per vial capped in a desiccated N2 atmosphere. They are shipped at ambient temperature and stored at room temperature.

Locus/Target
The BCR/ABL Lyophilized Cell Controls can be used as assay controls and validation material with assays that identify BCR/ABL t(9;22) transcripts. There should be a two-log difference in the measured levels of BCR/ABL transcript present in the 10-1 and 10-3 controls.

BCR/ABL b3a2 Lyophilized Cell Controls
BCR/ABL b2a2 Lyophilized Cell Controls
BCR/ABL e1a2 Lyophilized Cell Controls

DNA sensitivity panels are 6 member panels that consist of 100% clonal DNA extracted from a clonal cell line and 30%, 20%, 10%, 5% and 1% dilutions of the clonal DNA diluted (v/v) into our standard negative template control DNA, IVS-0000 Polyclonal Control DNA, cat# 4-092-0010.

View DNA Sensitivity Panels

RNA sensitivity panels are 7 member panels that consist of 100% clonal RNA extracted from a clonal cell line and 10-1, 10-2, 10-3, 10-4, 10-5, and 10-6 dilutions of the clonal RNA diluted (v/v) into our standard negative template control RNA, IVS-0035 Clonal Control RNA.

View RNA Sensitivity Panels

Our proficiency panels consist of dilutions of positive and negative DNA or RNA template controls. Each proficiency panel is designed to work with a specific assay.

Proficiency Panel for the BCR/ABL t(9;22) Translocations

Proficiency Panel for the PML/RARa t(15;17) Translocations